Research conducted investigated the efficiency of hemoglobin digestion in its native and denatured form using free and immobilized chymotrypsin enzyme. Goals were to determine an optimal enzyme-to-substrate ratio to digest hemoglobin in its denatured and native form using free and immobilized chymotrypsin enzyme, and use findings from the separation procedure of the capillary electrophoresis instrument to discover differences of crosslinking reagents—formaldehyde and glutaraldehyde, denatured vs. native hemoglobin, and free vs. immobilized chymotrypsin (CT).
